Table 1 SNPs and their frequencies detected in the pfk13 gene after amplification and sequencing
Codon | Sequence wild type (nt) | AA wild type | Sequence mutant allele (nt) | AA mutant | Mutation | Number (n) | Frequency (%) |
|---|---|---|---|---|---|---|---|
434 | ttt | F | att | I | F434I | 5 | 6.17% |
443 | cca | P | ccc | P | P443P | 1 | 1.23% |
447 | tgt | C | gat | D | C447D | 1 | 1.23% |
447 | tgt | C | tat | Y | C447Y | 1 | 1.23% |
449 | ggt | G | gat | D | G449D | 1 | 1.23% |
451 | ttt | F | ttg | L | F451L | 1 | 1.23% |
458 | aat | N | gat | D | N458D | 1 | 1.23% |
461 | gaa | E | aaa | K | E461K | 1 | 1.23% |
469 | tgc | C | tgt | C | C469C | 1 | 1.23% |
475 | cct | P | ccc | P | P475P | 1 | 1.23% |
478 | acc | T | gcc | A | T478A | 2 | 2.47% |
478 | acc | T | acg | T | T478T | 1 | 1.23% |
486 | gct | A | ggt | G | A486G | 1 | 1.23% |
494 | gtt | V | att | I | V494I | 1 | 1.23% |
499 | aac | N | aat | N | N499N | 1 | 1.23% |
507 | gaa | E | aaa | K | E507K | 1 | 1.23% |
508 | act | T | agt | S | T508S | 1 | 1.23% |
509 | gag | E | gaa | E | E509E | 1 | 1.23% |
512 | gat | D | aat | N | D512N | 1 | 1.23% |
513 | cgt | R | tgt | C | R513C | 1 | 1.23% |
515 | aga | R | aaa | K | R515K | 1 | 1.23% |
516 | gat | D | aat | N | D516N | 1 | 1.23% |
527 | cct | P | ctt | L | P527L | 1 | 1.23% |
535 | acg | T | act | T | T535T | 1 | 1.23% |
536 | tca | S | cca | P | S536P | 1 | 1.23% |
542 | tgt | C | cgt | R | C542R | 1 | 1.23% |
546 | tat | Y | tac | Y | Y546Y | 1 | 1.23% |
548 | ggc | G | gcc | A | G548A | 1 | 1.23% |
553 | ccg | P | cag | Q | P553Q | 1 | 1.23% |
553 | ccg | P | tcg | S | P553S | 1 | 1.23% |
556 | gaa | E | aaa | K | E556K | 3 | 3.70% |
557 | gca | A | gcg | A | A557A | 1 | 1.23% |
557 | gca | A | aca | T | A557T | 1 | 1.23% |
564 | gca | A | aca | T | A564T | 1 | 1.23% |
569 | gca | A | aca | T | A569T | 1 | 1.23% |
573 | acc | T | gcc | A | T573A | 1 | 1.23% |
578 | gct | A | act | T | A578T | 1 | 1.23% |
578 | gct | A | tct | S | A578S | 5 | 6.17% |
580 | tgt | C | tat | Y | C580Y | 1 | 1.23% |
589 | gtc | V | gtg | V | V589V | 1 | 1.23% |
595 | ggt | G | gat | D | G595D | 1 | 1.23% |
613 | caa | Q | cat | H | Q613H | 2 | 2.47% |
614 | ttt | F | ctt | L | F614L | 1 | 1.23% |
623 | agt | S | aat | N | S623N | 1 | 1.23% |
626 | gca | A | aca | T | A626T | 1 | 1.23% |
627 | gct | A | act | T | A627T | 1 | 1.23% |
630 | tac | Y | tat | Y | Y630Y | 2 | 2.47% |
638 | gga | G | ggg | G | G638G | 1 | 1.23% |
646 | ata | I | atg | M | I646M | 1 | 1.23% |
652 | caa | Q | taa | END | Q652END | 1 | 1.23% |
653 | tat | Y | ttt | F | Y653F | 1 | 1.23% |
655 | cca | P | tca | S | P655S | 1 | 1.23% |
657 | aat | N | agt | S | N657S | 1 | 1.23% |
661 | caa | Q | cca | P | Q661P | 1 | 1.23% |
668 | gag | E | aag | K | E668K | 1 | 1.23% |
668 | gag | E | cag | Q | E668Q | 1 | 1.23% |
668 | gag | E | gaa | E | E668E | 1 | 1.23% |
677 | aca | T | ata | I | T677I | 1 | 1.23% |
687 | gga | G | ggc | G | G687G | 1 | 1.23% |
688 | gaa | E | caa | Q | E688Q | 1 | 1.23% |
690 | ggc | G | ggt | G | G690G | 1 | 1.23% |
692 | gtt | V | att | I | V692I | 4 | 4.94% |
692 | gtt | V | ctt | L | V692L | 1 | 1.23% |
699 | ttt | F | ttc | F | F699F | 1 | 1.23% |
702 | gat | D | aaa | K | D702K | 1 | 1.23% |