Fig. 3

Candidate marker R515K. Electropherogram of the forward (a) and reverse complement (c) sequences with BioEdit 7.2 software. Alignment of the forward, reverse complement and consensus sequences compared to the reference P. falciparum 3D7 sequence (b), where the N nucleotide (highlighted with boxes) corresponds to the mixed peak with the mutant (A) and the wild-type (G) nucleotides, with almost the same intensity. The sequence alignment images (b) displays the AGA triplet (wild type) in the reference sequence, while the forward, reverse complement and consensus sequences shows the substitution of G with the mix A/G