Fig. 2

Validate marker C580Y detected in one sample. Electropherogram of the forward (a) and reverse complement (c) sequences with BioEdit 7.2 software. Alignment of the forward, reverse complement and consensus sequences compared to the reference P. falciparum 3D7 sequence (b). The sequence alignment images (b) displays the TGT triplet (wild type) in the reference sequence, while the forward, reverse complement and consensus sequences shows the substitution of G with A. Nucleotides corresponding to the TAT (mutant codon) are highlighted with boxes